Name: Anti-GPR120/FFAR4 (extracellular) Antibody
Brand: Alomone Labs
SKU: AFR-014

Overview

Cat #: AFR-014

Alternative Name G-protein coupled receptor 120, Free fatty acid receptor 4, FFA4 Receptor, G-protein coupled receptor GT01, Omega-3 fatty acid receptor 1, O3far1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RTHFPFFSDVKGDHR, corresponding to amino acid residues 22-36 of mouse FFAR4 (Accession Q7TMA4). Extracellular, N-terminus.

Accession (Uniprot) Number Q7TMA4

Gene ID 107221

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical; human – 14/15 amino acid residues identical.

RRID AB_2756590.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat lung and tongue, human U-87 astrocytoma and colorectal adenocarcinoma (Colo 205) cell lysates, mouse MS1 endothelial cell and colon lysates (1:200-1:1000).
Western blot analysis of rat lung (lanes 1 and 3), rat tongue (lanes 2 and 4), human U-87 astrocytoma cells (lanes 5 and 7) and mouse MS1 endothelial cells (lanes 6 and 8) lysates:
1,2,5,6. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
3,4,7,8. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).
Western blot analysis of mouse colon (lanes 9 and 11) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 10 and 12) lysates:
9,10. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).
11,12. Anti-GPR120/FFAR4 (extracellular) Antibody, preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).

Indirect flow cytometry

Human THP-1 monocytic leukemia cells (2.5 μg/0.5x10⁶ cells).

Scientific background

Free fatty acid receptor 4 (FFAR4, GPR120), is a member of the rhodopsin family of 7-transmembrane domain G-protein coupled receptors (GPCRs). GPR120/FFAR4 is activated by long chain fatty acids. The protein is a potential therapeutic target for the treatment of type 2 diabetes mellitus (T2DM). GPR120 is associated to insulin sensitizing, anti-inflammatory, and fat metabolism. Evidence shows that GPR120 may be involved in the development of obesity in mice and humans^1-4. GPR120 regulates various physiological processes, including gut hormone secretion, islet function, osteoclastogenesis, anti-inflammation, and adipogenesis^2-4.

GPR120 binds to omega-3 fatty acids and stabilizes the metabolic homeostasis through a cascade of physiological activities. GPR120 exerts its physiological effects through one of two pathways that involves either G_αq or β-arrestin^2,3.

GPR120 has been shown to be ubiquitously expressed and is primarily detected in the intestine, adipocytes, and pro-inflammatory macrophages^1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014) is the original antigen used for immunization during Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014) generation. The blocking peptide binds and ‘blocks’ Anti-GPR120/FFAR4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014)

Shipping and Ordering Information

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Certificate of Analysis

Anti-GPR120/FFAR4 (extracellular) Antibody