Name: Anti-GPR126/ADGRG6 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-056

Overview

Cat #: AGR-056

Alternative Name Adhesion G-Protein Coupled Receptor G6, G-Protein Coupled Receptor 126, Developmentally Regulated G-Protein-Coupled Receptor, DREG, Vascular Inducible G Protein-Coupled Receptor, VIGR

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KDPVQIKIKHTRTQE, corresponding to amino acid residues 753 - 767 of mouse GPR126 (Accession Q86SQ4). Extracellular, N-terminus.

Accession (Uniprot) Number Q86SQ4

Gene ID 57211

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human - identical.

RRID AB_2876821

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of mouse lung lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
1, 2. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
3, 4. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) (#BLP-GR056).
Western blot analysis of rat brain membranes:
1. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
2. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) (#BLP-GR056).
Western blot analysis of human LNCaP prostate carcinoma cell line lysate (lanes 1 and 5), human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human K562 erythroleukemia cell line lysate (lanes 4 and 8):
1-4. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
5-8. Anti-GPR126/ADGRG6 (extracellular) Antibody, preincubated with blocking peptide.

References

Langenhan, T. et al. (2013) Sci. Signal. 6, 276.
Sun, P. et al. (2020) Sci. Adv. 6, 12.
Küffer, A. et al. (2016) Nature 536, 464.
Paavola, K.J. et al. (2014) Sci. Signal. 7, ra76.

Scientific background

GPR126 is an adhesion G-protein coupled receptor; the second largest subgroup of GPCRs¹. The defining characteristic of the adhesion GPCR family is their extraordinarily large N-terminal extracellular domain important to communicate with extracellular ligands and mediate adhesive functions².

GPR126 is implicated in an increasing number of developmental defects: skeletal defects, including shortened height, adolescent idiopathic scoliosis, arthrogryposis multiplex congenital, and periodontitis. Other studies reveal that GPR126 is essential for myelination of axons in the peripheral nervous system and the heart².

Known ligands of GPCR126 are type IV collagen (COLIV) in peripheral nerves and inner ear development, Laminin-211 during Schwann cell development, and prion protein in myelination³, suggesting that GPR126 could use different signaling pathways in different tissues⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize ADGR6 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056) is the original antigen used for immunization during Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056) generation. The blocking peptide binds and ‘blocks’ Anti-GPR126/ADGRG6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR126/ADGRG6 (extracellular) Blocking Peptide (#BLP-GR056)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-GPR126/ADGRG6 (extracellular) Antibody