Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
Mouse lung and heart lysates; rat brain membranes; human LNCaP prostate carcinoma, MCF-7 breast adenocarcinoma, Malme-3M melanoma, and human K562 erythroleukemia cell lysates (1:200-1:1000).
Western blot analysis of mouse lung lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):
GPR126 is an adhesion G-protein coupled receptor; the second largest subgroup of GPCRs1. The defining characteristic of the adhesion GPCR family is their extraordinarily large N-terminal extracellular domain important to communicate with extracellular ligands and mediate adhesive functions2.
GPR126 is implicated in an increasing number of developmental defects: skeletal defects, including shortened height, adolescent idiopathic scoliosis, arthrogryposis multiplex congenital, and periodontitis. Other studies reveal that GPR126is essential for myelination of axons in the peripheral nervous system and the heart2.
Known ligands of GPCR126 are type IV collagen (COLIV) in peripheral nerves and inner ear development, Laminin-211 during Schwann cell development, and prion protein in myelination3, suggesting that GPR126 could use different signaling pathways in different tissues4.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of mouse lung lysate (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):1, 2. Anti-GPR126/ADGRG6 (extracellular) Antibody (#AGR-056), (1:200).
