Name: Anti-GPR142 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-082

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Overview

Cat #: AGR-082

Alternative Name G-Protein Coupled Receptor 142, Probable G-Protein Coupled Receptor 142, PGR2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)APVHRDWRVHLALD, corresponding to amino acid residues 291 - 304 of mouse GPR142 (Accession Q7TQN9). Extracellular, 3^rd loop.

Accession (Uniprot) Number Q7TQN9

Gene ID 217302

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, human – identical

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse pancreas lysate (lanes 1 and 3) and rat pancreas lysate (lanes 2 and 4):
1-2. Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:400).
3-4. Anti-GPR142 (extracellular) Antibody, preincubated with GPR142 (extracellular) Blocking Peptide (BLP-GR082).
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
1-2. Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:400).
3-4. Anti-GPR142 (extracellular) Antibody, preincubated with GPR142 (extracellular) Blocking Peptide (BLP-GR082).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
1-3. Anti-GPR142 (extracellular) Antibody (#AGR-082), (1:400).
4-6. Anti-GPR142 (extracellular) Antibody, preincubated with GPR142 (extracellular) Blocking Peptide (BLP-GR082).

Scientific background

G-protein-coupled receptors (GPCRs) are the largest family of membrane proteins and mediate a myriad of physiological and pathophysiological signaling processes. Of these receptors, all are seven membrane spanning receptors but not all are G protein binding but it is convenient to refer to the receptors as GPCRs¹.

The GPR142 is a GPCR selectively activated by aromatic amino acids and share 33% amino acid identity with GPR139. L-tryptophan representing one of the most potent ligands, having an EC50 values of 0.2 – 1 mM². GPR142 is expressed in both rodent and human pancreatic beta cells. Moreover, it was found to be implicated in the regulation of insulin, thereby having a crucial role in Type II diabetes management³.

Moreover, GPR142 is highly expressed in immune cells and its ligand L-tryptophan and metabolite serotonin are known to take part in inflammation process. It was shown that mice lacking GPR142 gene are more resilient to anti-type II collagen (CII) antibody-induced arthritis however the exact mechanism is currently unknown⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

GPR142 (extracellular) Blocking Peptide (#BLP-GR082) is the original antigen used for immunization during Anti-GPR142 (extracellular) Antibody (#AGR-082) generation. The blocking peptide binds and ‘blocks’ Anti-GPR142 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR142 (extracellular) Blocking Peptide (#BLP-GR082)

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Certificate of Analysis

Anti-GPR142 (extracellular) Antibody