Name: Anti-GPR17 Antibody
Brand: Alomone Labs
SKU: AGR-048

Overview

Cat #: AGR-048

Alternative Name G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)RQGPRIEKHLKNK, corresponding to amino acid residues 220-232 of rat GPR17 (Accession Q09QM4). Intracellular, 3^rd loop.

Accession (Uniprot) Number Q09QM4

Gene ID 767613

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical.

RRID AB_2756637.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for human samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1,2. Anti-GPR17 Antibody (#AGR-048), (1:200).
3,4. Anti-GPR17 Antibody, preincubated with GPR17 Blocking Peptide (#BLP-GR048).

Immunohistochemistry

Expression of GPR17 in rat parietal cortex
Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR17 Antibody (#AGR-048), (1:300), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. GPR17 immunoreactivity (red) appears in cortical cells (arrows). Cell nuclei are stained with DAPI (blue).

References

He, Y. et al. (2018) Biosci. Rep. 38, 1.
Bonfanti, E. et al. (2017) Cell Death Dis. 8, 6.
Hennen, S. et al. (2013) Sci. Signal. 6, 298.
Rosenbaum, D.M. et al. (2009) Nature 459, 356.

Scientific background

GPR17 is an orphan receptor that can be double-activated by uracil and cysteinyl-leukotriene (CysLT). It is phylogenetically related to both purinergic P2Y and CysLT receptors. GPR17 belongs to the superfamily of G-protein coupled receptors (GPCRs)⁴.

GPR17 expression is highly detected in the brain, kidney, and heart. In the brain, GPR17 is mainly distributed in white matter, gray matter, subependymal zone, and corpus callosum¹.

Studies have shown that GPR17 receptor plays an important role in spinal cord injury, myelin sheath injury, cerebral ischemic injury, where its expression is extremely upregulated^1,2.

GPR17 activation promotes oligodendrocyte differentiation and progression toward mature myelin-forming cells. Thus, GPR17 modulators may be used as therapeutic tools to treat pathologies associated with myelin repair in CNS demyelinating diseases³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR17 Antibody (#AGR-048) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR17 from rat and mouse samples. It is unlikely to recognize the protein from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR17 Blocking Peptide (#BLP-GR048) is the original antigen used for immunization during Anti-GPR17 Antibody (#AGR-048) generation. The blocking peptide binds and ‘blocks’ Anti-GPR17 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR17 Blocking Peptide (#BLP-GR048)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-GPR17 Antibody