Name: Anti-GPR171 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-054

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Overview

Cat #: AGR-054

Alternative Name G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171 (Accession Q8BG55). 2^nd extracellular loop.

Accession (Uniprot) Number Q8BG55

Gene ID 229323

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – 14/15 amino acid residues identical; rat – 13/15 amino acid residues identical.

RRID AB_2876840

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Rat and mouse brain membranes; human MEG-01 megakaryoblastic leukemia cell, and Jurkat T-cell leukemia cell lysates (1:200-1:1000).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1, 2. Anti-GPR171 (extracellular) Antibody (#AGR-054), (1:200).
3, 4. Anti-GPR171 (extracellular) Antibody, preincubated with GPR171 (extracellular) Blocking Peptide (#BLP-GR054).

Indirect flow cytometry

Human live intact Jurkat T-cell leukemia cells (5 µg).

Scientific background

G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN; one of the most abundant neuropeptides in the brain that is derived from ProSAAS¹. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor^2,3.

BigLEN–GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area^4-6.

This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders^4,5.

Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions⁶.

GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

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Anti-GPR171 (extracellular) Antibody