Name: Anti-GPR171 (extracellular)-PE Antibody
Brand: Alomone Labs
SKU: AGR-054-PE

Overview

Cat #: AGR-054-PE

Alternative Name G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171 (Accession Q8BG55). 2^nd extracellular loop.

Accession (Uniprot) Number Q8BG55

Gene ID 229323

Homology Mouse, human – 14/15 amino acid residues identical; rat – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Isotype Rabbit IgG

Clone R-Phycoerythrin (R-PE)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-054), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of GPR171 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE (#RIC-001-PE).
^___ Cells + Anti-GPR171 (extracellular)-PE Antibody (#AGR-054-PE), (2.5µg).
Cell surface detection of GPR171 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-PE (#RIC-001-PE).
^___ Cells + Anti-GPR171 (extracellular)-PE Antibody (#AGR-054-PE), (5µg).

References

Fricker, L.D. (2010) Mol. Biosyst. 6, 1355.
Schöneberg, T. et al. (2007) Purinergic Signal. 3, 255.
Wardman, J.H. et al. (2016) Sci. Signal. 9, ra55.
Gomes, I. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 16211.
Bobeck, E.N. et al. (2017) Neuropsychopharmacology 42, 2527.
McDermott, M.V. et al. (2019) J. Pharmacol. Exp. Ther. 371, 56.

Scientific background

G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN; one of the most abundant neuropeptides in the brain that is derived from ProSAAS¹. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor^2,3.

BigLEN–GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area^4-6.

This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders^4,5.

Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions⁶.

GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR171 (extracellular) Antibody (#AGR-054) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, and live cell imaging applications. It has been designed to recognize GPR171 from human, mouse and rat samples.

Anti-GPR171 (extracellular)-PE Antibody (#AGR-054-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-PE (#RIC-001-PE), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Phycoerythrin (PE) fluorophore.

Rabbit IgG Isotype Control-PE (#RIC-001-PE)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-GPR171 (extracellular)-PE Antibody