Name: Anti-GPR174 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGR-060-F

Overview

Cat #: AGR-060-F

Alternative Name G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174 (Accession Q9BXC1). 2^nd extracellular loop.

Accession (Uniprot) Number Q9BXC1

Gene ID 84636

Homology Human – 15/16 amino acid residues identical; mouse, rat – 13/16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-060), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of GPR174 by direct flow cytometry in live intact mouse TK-1
T-cell lymphoma cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (5µg).
Cell surface detection of GPR174 by direct flow cytometry in live intact mouse WEHI-231 B lymphocytes cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (2.5µg).
Cell surface detection of GPR174 by direct flow cytometry in live intact human Jurkat
T-cell leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (5µg).

References

Kihara, Y. et al. (2014) Br. J. Pharmacol. 171, 3575.
Sugita, K. et al. (2013) Biochem. Biophys. Res. Commun. 430, 190.
Zhao, S.X. et al. (2013) Hum. Mol. Genet. 22, 3347.
Barnes, M.J. and Cyster, J.G. (2018) Immunol. Cell Biol. 96, 439.

Scientific background

GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals¹.

Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation².

GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism³ and as a negative regulator of T-cells activation⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR174 (extracellular) Antibody (#AGR-060) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR174 from rat, mouse and human samples.

Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR174 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Shipping and Ordering Information

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Anti-GPR174 (extracellular)-FITC Antibody