Overview
Alternative Name G-protein coupled receptor 174, LYPSR3, GPCR17, FKSG79
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)DTSGNRTK(S)FVDLPTR, corresponding to amino acid residues 160 - 175 of human GPR174 (Accession Q9BXC1). 2nd extracellular loop.
Accession (Uniprot) Number Q9BXC1
Gene ID 84636
Homology Human – 15/16 amino acid residues identical; mouse, rat – 13/16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-060), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
Cell surface detection of GPR174 by direct flow cytometry in live intact mouse TK-1
T-cell lymphoma cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (5µg).
Cell surface detection of GPR174 by direct flow cytometry in live intact mouse WEHI-231 B lymphocytes cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (2.5µg).
Cell surface detection of GPR174 by direct flow cytometry in live intact human Jurkat
T-cell leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-GPR174 (extracellular)-FITC Antibody (#AGR-060-F), (5µg).
Scientific background
GPR174 (LysoPS, LYPSR3), is one of three lysophosphatidyl serine receptors, belonging to the superfamily of G-protein coupled receptors. Lysophospholipids are a diverse group of small, membrane-derived phospholipids that act as extracellular signals1.
Activation of GPR174 leads to an increase in cAMP cellular levels, leading to cellular morphological changes, cell to cell adhesion, and delays in cell proliferation2.
GPR174 is reported to be a genetic risk locus for Graves disease, an autoimmune disorder that causes hyperthyroidism3 and as a negative regulator of T-cells activation4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
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