Name: Anti-GPR34 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGR-055-F

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Overview

Cat #: AGR-055-F

Alternative Name G-protein coupled receptor 34

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CFHYRDRHNAKGE, corresponding to amino acid residues 197-209 of mouse GPR34 (Accession Q9R1K6). 2^nd extracellular loop.

Accession (Uniprot) Number Q9R1K6

Gene ID 23890

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human, rat – 12/13 amino acid residues identical.

RRID AB_2876804

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-055), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Mouse BV-2 microglia cells; human THP-1 monocytic leukemia cells (2.5 µg).
Cell surface detection of GPR34 in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GPR34 (extracellular)-FITC Antibody (#AGR-055-F), (2.5 µg).

Scientific background

The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y₁₂-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)¹.

The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus².

GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system–derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies show that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype^2,3,4.

GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages; GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients; GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 07/05/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Certificate of Analysis

Anti-GPR34 (extracellular)-FITC Antibody