Name: Anti-GPR37 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-049

Overview

Cat #: AGR-049

Alternative Name G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, Endothelin B receptor-like protein 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RVRLKNPFYPLTQE, corresponding to amino acid residues 234-247 of rat GPR37 (Accession Q9QYC6). Extracellular, N-terminus.

Accession (Uniprot) Number Q9QYC6

Gene ID 117549

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, wb

May also work in: ic*, ih*, ip*, lci*

Western blot

Mouse and rat brain lysates (1:200-1:1000).
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-GPR37 (extracellular) Antibody (#AGR-049), (1:200).
3,4. Anti-GPR37 (extracellular) Antibody, preincubated with GPR37 (extracellular) Blocking Peptide (#BLP-GR049).

Indirect flow cytometry

Human THP-1 monocytic leukemia cells (2.5 µg).

Scientific background

GPR37 or parkin-associated endothelin-like receptor, is an orphan G-protein coupled receptor (GPCR) almost exclusively expressed in the central nervous system. High levels of the protein are detected in the cerebellum, corpus callosum, caudate nucleus, putamen, substantia nigra, and hippocampus.

GPR37 is a substrate of the E3 ubiquitin ligase parkin and acts as a receptor for prosaposin. The protein affects oligodendrocyte differentiation and myelination during development^1,2.

Studies show that GPR37 is heavily involved in the pathogenesis of Parkinson's disease. Missense mutations of GPR37 substrate E3 ubiquitin ligase parkin result in the accumulation of GPR37 in the brain of Parkinson's disease patients^1,2_.In addition, GPR37 levels are up‐regulated in the brains of juvenile Parkinson disease patients and down‐regulated in the amygdala and hippocampus of major depressive disorder patients³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

GPR37 (extracellular) Blocking Peptide (#BLP-GR049) is the original antigen used for immunization during Anti-GPR37 (extracellular) Antibody (#AGR-049) generation. The blocking peptide binds and ‘blocks’ Anti-GPR37 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR37 (extracellular) Blocking Peptide (#BLP-GR049)

Shipping and Ordering Information

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Certificate of Analysis

Anti-GPR37 (extracellular) Antibody