Name: Anti-GPR56 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-047

Overview

Cat #: AGR-047

Alternative Name G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56 (Accession Q8K3V3). Extracellular, N-terminus.

Accession (Uniprot) Number Q8K3V3

Gene ID 260326

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – 13/14 amino acid residues identical; human – 11/14 amino acid residues identical.

RRID AB_2756636.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
1,2. Anti-GPR56 (extracellular) Antibody (#AGR-047), (1:200).
3,4. Anti-GPR56 (extracellular) Antibody, preincubated with GPR56 (extracellular) Blocking Peptide (#BLP-GR047).
Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 3) and human NK-92 natural killer (lanes 2 and 4) cell line lysates:
1,2. Anti-GPR56 (extracellular) Antibody (#AGR-047), (1:200).
3,4. Anti-GPR56 (extracellular) Antibody, preincubated with GPR56 (extracellular) Blocking Peptide (#BLP-GR047).

Indirect flow cytometry

Cell surface detection of GPR56 in live intact human HL-60 promyelocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-GPR56 (extracellular) Antibody (#AGR-047), 5 µg + goat-anti-rabbit-FITC.

References

Moreno, M. et al. (2017) Cell Rep. 21, 2183.
Aust, G. et al. (2016) Handb. Exp. Pharmacol. 234, 369.
Singer, K. et al. (2013) Mol. Neurobiol. 47, 186.

Scientific background

GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion^1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present^1-³.

Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo^1-3.

Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain^2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR56 (extracellular) Antibody (#AGR-047) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR56 (extracellular) Blocking Peptide (#BLP-GR047) is the original antigen used for immunization during Anti-GPR56 (extracellular) Antibody (#AGR-047) generation. The blocking peptide binds and ‘blocks’ Anti-GPR56 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR56 (extracellular) Blocking Peptide (#BLP-GR047)

Shipping and Ordering Information

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Anti-GPR56 (extracellular) Antibody