Name: Anti-GPR68 (OGR1) (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-042

Overview

Cat #: AGR-042

Alternative Name Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68 (Accession Q8BFQ3). 2^nd extracellular loop.

Accession (Uniprot) Number Q8BFQ3

Gene ID 238377

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse, human – 14/15 amino acid residues identical.

RRID AB_2756632.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat kidney lysates (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4):
1,2. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
3,4. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042).
Western blot analysis of rat heart membranes:
1. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
2. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042).

Indirect flow cytometry

Cell surface detection of GPR68 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (2.5 µg) + goat-anti-rabbit-FITC.

References

Thompson, M.D. et al. (2005) Crit. Rev. Clin. Lab Sci. 42, 311.
Xu, Y. and Casey, G. (1996) Genomics. 35, 397.
Zaslavsky, A. et al. (2006) Biochim. Biophys. Acta. 1761, 1200.
Mohebbi, N. et al. (2012) Cell. Physiol. Biochem. 29, 313.

Scientific background

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus¹. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR4².

GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP₃) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H⁺-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an epitope of the mouse ovarian cancer G-protein coupled receptor 1. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR68 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042) is the original antigen used for immunization during Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) generation. The blocking peptide binds and ‘blocks’ Anti-GPR68/OGR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042)

Shipping and Ordering Information

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Anti-GPR68 (OGR1) (extracellular) Antibody