Name: Anti-GPR68 (OGR1) (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-042

Overview

Cat #: AGR-042

Alternative Name Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68 (Accession Q8BFQ3). 2^nd extracellular loop.

Accession (Uniprot) Number Q8BFQ3

Gene ID 238377

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse, human – 14/15 amino acid residues identical.

RRID AB_2756632.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Rat and mouse kidney lysates and rat heart membranes (1:200-1:1000).
Western blot analysis of rat kidney lysates (lanes 1 and 3) and mouse kidney membranes (lanes 2 and 4):
1,2. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
3,4. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042).
Western blot analysis of rat heart membranes:
1. Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042), (1:200).
2. Anti-GPR68 (OGR1) (extracellular) Antibody, preincubated with GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042).

Indirect flow cytometry

Human THP-1 monocytic leukemia cells.

Scientific background

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus¹. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR4².

GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP₃) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H⁺-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042) is the original antigen used for immunization during Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) generation. The blocking peptide binds and ‘blocks’ Anti-GPR68/OGR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR68/OGR1 (extracellular) Blocking Peptide (#BLP-GR042)

Shipping and Ordering Information

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Certificate of Analysis

Anti-GPR68 (OGR1) (extracellular) Antibody