Name: Anti-GPR68 (OGR1) (extracellular)-APC Antibody
Brand: Alomone Labs
SKU: AGR-042-APC

Overview

Cat #: AGR-042-APC

Alternative Name Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68 (Accession Q8BFQ3). 2^nd extracellular loop

Accession (Uniprot) Number Q8BFQ3

Gene ID 238377

Homology Rat, mouse, human – 14/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Allophycocyanin (APC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-042), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of GPR68 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5 µg).
Cell surface detection of GPR68 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5 µg).
Multicolor flow cytometry with GPR68 and GPR132
Human THP-1 monocytic leukemia cell line was labeled by adding Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5µg) and Anti-Human GPR132/G2A (extracellular)-FITC Antibody (#AGR-046-F), (5µg).
Multicolor flow cytometry with GPR68 and GPR65
Human THP-1 monocytic leukemia cell line was labeled by adding Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5µg) and Anti-GPR65 (TDAG8) (extracellular)-PE Antibody (#AGR-043-PE), (5µg)

References

Thompson, M.D. et al. (2005) Crit. Rev. Clin. Lab Sci. 42, 311.
Xu, Y. and Casey, G. (1996) Genomics. 35, 397.
Zaslavsky, A. et al. (2006) Biochim. Biophys. Acta. 1761, 1200.
Mohebbi, N. et al. (2012) Cell. Physiol. Biochem. 29, 313.

Scientific background

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus¹. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR4².

GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP₃) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H⁺-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR68 (OGR1) (extracellular) Antibody (#AGR-042) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize GPR68 from mouse, rat and human samples.

Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC) is directly conjugated to Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

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Anti-GPR68 (OGR1) (extracellular)-APC Antibody