Name: Anti-GPR68 (OGR1) (extracellular)-APC Antibody
Brand: Alomone Labs
SKU: AGR-042-APC

Overview

Cat #: AGR-042-APC

Alternative Name Acid-sensing G-protein coupled receptor 68, Ovarian cancer G-protein coupled receptor 1, Sphingosylphosphorylcholine receptor, AI2A6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)HRV(S)FEHYPIQAWQR, corresponding to amino acid residues 169-183 of mouse GPR68 (Accession Q8BFQ3). 2^nd extracellular loop

Accession (Uniprot) Number Q8BFQ3

Gene ID 238377

Homology Rat, mouse, human – 14/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Allophycocyanin (APC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-042), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of GPR68 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5 µg).
Cell surface detection of GPR68 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype control-APC (#RIC-001-APC).
^___ Cells + Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5 µg).
Multicolor flow cytometry with GPR68 and GPR132
Human THP-1 monocytic leukemia cell line was labeled by adding Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5µg) and Anti-Human GPR132/G2A (extracellular)-FITC Antibody (#AGR-046-F), (5µg).
Multicolor flow cytometry with GPR68 and GPR65
Human THP-1 monocytic leukemia cell line was labeled by adding Anti-GPR68 (OGR1) (extracellular)-APC Antibody (#AGR-042-APC), (5µg) and Anti-GPR65 (TDAG8) (extracellular)-PE Antibody (#AGR-043-PE), (5µg)

Scientific background

All GPCRs, including GPR68, are single peptides possessing seven transmembrane domains with an extracellular N-terminus and an intracellular C-terminus¹. GPR68 is expressed in several tissues including the spleen, testis, small bowel, brain, heart, lung and kidneys. It shares strong homology (49-54%) with the human receptor GPR4².

GPR68 is proton sensitive in the pH range of 6.8-7.8 and acts as a proton activated receptor. GPR68 stimulates inositol triphosphate (IP₃) formation thereby releasing intracellular calcium and activating the mitogen activated protein kinase/extracellular signal-regulated (ERK) kinase cascade. In addition, GPR68 receptor regulates NHE3 and H⁺-ATPase, in response to extracellular pH changes in cultured HEK 293 renal cells. This activation requires a cluster of histidine residues in the extracellular domains of the receptor and protein-kinase C (PKC) activation⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 03/01/2024

Specifications

Citations

Applications

Scientific Background

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Anti-GPR68 (OGR1) (extracellular)-APC Antibody