Name: Anti-GPR84 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-052

Overview

Cat #: AGR-052

Alternative Name G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5). 2^nd extracellular loop.

Accession (Uniprot) Number Q9NQS5

Gene ID 53831

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human – 14/15 amino acid residues identical; mouse, rat – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6):
1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).
Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human U-87 MG glioblastoma (lanes 3 and 6) cell line lysates:
1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).

Immunohistochemistry

Expression of GPR84 in mouse fornix in a kainic acid-induced model of temporal lobe epilepsy
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. In untreated mouse sections GPR84 immunoreactivity (green) is detected in astrocyte profiles (arrows). B. GPR84 staining (green) in mouse sections obtained four days post kainate-induced seizures, shows an increase in number and size of astrocyte profiles stained for GPR84 (arrows). Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of GPR84 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-GPR84 (extracellular) Antibody (#AGR-052), (2.5 µg) + goat-anti-rabbit-FITC.

References

Pillaiyar, T. et al. (2018) ACS Omega 3, 3365.
Alvarez-Curto, E. and Milligan, G. (2016) Biochem. Pharmacol. 114, 3.
Suzuki, M. et al. (2013) J. Biol. Chem. 288, 10684.

Scientific background

GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive G_i/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer^1-3.

GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.

Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR84 (extracellular) Antibody (#AGR-052) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

GPR84 (extracellular) Blocking Peptide (#BLP-GR052) is the original antigen used for immunization during Anti-GPR84 (extracellular) Antibody (#AGR-052) generation. The blocking peptide binds and ‘blocks’ Anti-GPR84 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR84 (extracellular) Blocking Peptide (#BLP-GR052)

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Anti-GPR84 (extracellular) Antibody