Name: Anti-GPR84 (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-052

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Overview

Cat #: AGR-052

Alternative Name G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5). 2^nd extracellular loop.

Accession (Uniprot) Number Q9NQS5

Gene ID 53831

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human – 14/15 amino acid residues identical; mouse, rat – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Rat and mouse brain membranes, rat spleen membranes, human THP-1 monocytic leukemia cell, HL-60 promyelocytic leukemia cell and U-87 MG glioblastoma cell lysates (1:200-1:1000).
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6):
1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).
Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human U-87 MG glioblastoma (lanes 3 and 6) cell line lysates:
1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052), (1:200).
4-6. Anti-GPR84 (extracellular) Antibody, preincubated with GPR84 (extracellular) Blocking Peptide (#BLP-GR052).

Immunohistochemistry

Mouse brain sections (1:200).

Indirect flow cytometry

Mouse J774 macrophage cells (2.5 µg).

Scientific background

GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive G_i/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer^1-3.

GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.

Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

GPR84 (extracellular) Blocking Peptide (#BLP-GR052) is the original antigen used for immunization during Anti-GPR84 (extracellular) Antibody (#AGR-052) generation. The blocking peptide binds and ‘blocks’ Anti-GPR84 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

GPR84 (extracellular) Blocking Peptide (#BLP-GR052)

Shipping and Ordering Information

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Certificate of Analysis

Anti-GPR84 (extracellular) Antibody