Name: Anti-GPR84 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGR-052-F

Overview

Cat #: AGR-052-F

Alternative Name G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5). 2^nd extracellular loop.

Accession (Uniprot) Number Q9NQS5

Gene ID 53831

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human – 14/15 amino acid residues identical; mouse, rat – 13/15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label Fluorescein isothiocyanate (FITC).

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 μl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGR-052), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of GPR84 in live intact mouse BV-2 microglia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F), (5 µg).
Cell surface detection of GPR84 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F), (2.5 µg).

References

Pillaiyar, T. et al. (2018) ACS Omega 3, 3365.
Alvarez-Curto, E. and Milligan, G. (2016) Biochem. Pharmacol. 114, 3.
Suzuki, M. et al. (2013) J. Biol. Chem. 288, 10684.

Scientific background

GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive G_i/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer^1-3.

GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.

Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-GPR84 (extracellular) Antibody (#AGR-052) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

Anti-GPR84 (extracellular)-FITC Antibody (#AGR-052-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-GPR84 (extracellular)-FITC Antibody