Overview
- Peptide (C)RKSVTLRKMKQEIKE, corresponding to amino acid residues 963-977 of rat GRIP1 (Accession P97879). Intracellular, C-terminus.
Western blot analysis of mouse brain lysate:1. Anti-GRIP1 Antibody (#APZ-015), (1:200).
2. Anti-GRIP1 Antibody, preincubated with GRIP1 Blocking Peptide (#BLP-PZ015).
Western blot analysis of rat brain synaptosomal fraction:1. Anti-GRIP1 Antibody (#APZ-015), (1:200).
2. Anti-GRIP1 Antibody, preincubated with GRIP1 Blocking Peptide (#BLP-PZ015).
- Han, M. et al. (2017) Behav. Brain. Res. 321, 176.
- Modjeski, K.L. et al. (2016) PLoS ONE 11, e0160638.
- Modjeski, K.L. et al (2016) Am. J. Transplant 16, 1383.
GRIP1 (glutamate receptor interacting protein 1) is a neuron-enriched scaffolding protein that plays an important role in regulating synaptic trafficking, synaptic strength and synaptic organization and transmission of AMPA receptor 2 and 3 (GluA2/3) 1,2. GRIP1 structure contains 7 PDZ domains responsible for protein-protein interactions. GRIP1 forms homodimers that can bind and simultaneously organize and assemble protein complexes via PDZ domains1,2.
GRIP1 is abundantly expressed in glutamatergic and GABAergic synapses. In addition, GRIP1 expression can be found in muscle cells and in the epidermis1,3. Studies on GRIP1 knockout mice have revealed multi-organ developmental abnormalities. These mice die due to hemorrhage and hypovolemia in the embryonic or early post-natal period2.
Mutations in GRIP1 protein resulting in a gain-of-function phenotype are linked to autism, while neuronal-specific loss-of-function mice have prosocial behavior. Other point mutations have been associated with schizophrenia and congenital kidney disease2,3.
Anti-GRIP1 Antibody (#APZ-015) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GRIP1 from human, mouse, and rat samples.
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