Name: Guinea pig Anti-HCN4 Antibody
Brand: Alomone Labs
SKU: APC-052-GP

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Overview

Cat #: APC-052-GP

Alternative Name Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Lyophilized Powder yes

Type: Polyclonal

Host: Guinea pig

Reactivity: h, m, r

Immunogen

GST fusion protein with the sequence HGHLHDSAEE RRLIAEGDASPGEDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4). Intracellular, N-terminus.

Accession (Uniprot) Number Q9Y3Q4

Gene ID 59266

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rabbit - identical; rat - 35/37 amino acid residues identical; mouse - 33/37 amino acid residues identical.

RRID AB_2340957.

Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Guinea pig total IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Rat brain lysate (1:200).
Western blot analysis of rat brain lysate:
1. Guinea pig Anti-HCN4 Antibody (#APC-052-GP), (1:200).
2. Guinea pig Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).
Following a broad screen of secondary antibodies, the following was used for this application: #106-035-006 (Jackson ImmunoResearch).

Immunohistochemistry

Mouse thalamus. Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).

Scientific background

Hyperpolarization-activated cation currents (I_h) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.^1,2

The I_h currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.

Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.²

The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.²

The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.

HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .^4,5

mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.⁶

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 20/11/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

HCN4 Blocking Peptide (#BLP-PC052) is the original antigen used for immunization during Anti-HCN4 Antibody (#APC-052) generation. The blocking peptide binds and ‘blocks’ Anti-HCN4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

HCN4 Blocking Peptide (#BLP-PC052)

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Certificate of Analysis

Guinea pig Anti-HCN4 Antibody