Overview
- Peptide (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor (Accession P70174). 2nd extracellular loop.
Cell surface detection of Histamine H1 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (#AHR-006-F), (5µg)
Cell surface detection of Histamine H1 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (#AHR-006-F), (5µg)
- Shiroishi, M. and Kobayashi, T. (2016) Handb. Exp. Pharmacol. 241, 21.
- Hill, S.J. (1990) Pharmacol. Rev. 42, 45.
- Obara, I. et al. (2020) Br. J. Pharmacol. 177, 580.
Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.
H1 receptor is excitatory and couples to Gq‐type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.
H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.
H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.
Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006) is a highly specific antibody directed against an extracellular epitope of the mouse H1R. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize H1R from mouse, rat and human samples.
Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (#AHR-006-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize H1R from mouse, rat and human samples.
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