Name: Anti-Human CaV1.2 (CACNA1C) Antibody
Brand: Alomone Labs
SKU: ACC-022

Overview

Cat #: ACC-022

Alternative Name Human α_1C, Voltage-dependent L-type calcium channel subunit alpha-1C.

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide VNENTRMYIPEENHQ(C), corresponding to amino acid residues 2-15 of human Ca_V1.2 (exon 1B) (Accession Q13936). Intracellular, N-terminus.

Accession (Uniprot) Number Q13936

Gene ID 775

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat - 14/15 amino acid residues identical.

RRID AB_2039769.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity The antibody recognizes mouse Ca_V1.2, splice variants P22002-4 and P22002-5 of rat Ca_V1.2 and splice variant P15381-4 of rabbit Ca_V1.2.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, wb

May also work in: ifc*, ih*, ip*

Western blot

Western blot analysis of rat heart membranes:
1. Anti-Human-Ca_V1.2 (CACNA1C) Antibody (#ACC-022), (1:200).
2. Anti-Human-Ca_V1.2 (CACNA1C) Antibody, preincubated with Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022).
Mouse cortex, hippocampus, and cerebellum lysates (1:200) (White, J.A. et al. (2008) Learn. Mem. 15, 1.).

Immunocytochemistry

Rat nodose ganglion neurons in culture (1:15) (Pamidimukkala, J. and Hay, M. (2004) Brain Res. 1006, 215.).

References

Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
IUPHAR

Scientific background

All L-type calcium channels are encoded by one of the Ca_V1 channel genes. These channels play a major role as a Ca²⁺ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All Ca_V1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

While the Ca_V1.1 and Ca_V1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of Ca_V1.2 is ubiquitous and Ca_V1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the Ca_V1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Ca_V1.2 channel. Anti-Human Ca_V1.2 (CACNA1C) Antibody (#ACC-022) can be used in western blot and immunocytochemistry applications. It has been designed to recognize Ca_V1.2 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Mouse cortex, hippocampus, and cerebellum lyastes (1:200).
White, J.A. et al. (2008) Learn. Mem. 15, 1.

Immunocytochemistry citations

Rat nodose ganglion neurons in culture (1:15).
Pamidimukkala, J. and Hay, M. (2004) Brain Res. 1006, 215.

Specifications

Scientific Background

Specific Control Product

Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022) is the original antigen used for immunization during Anti-Human Ca_V1.2 (CACNA1C) Antibody (#ACC-022) generation. The blocking peptide binds and ‘blocks’ Anti-Human Cav1.2/CACNA1C primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human Cav1.2/CACNA1C Blocking Peptide (#BLP-CC022)

Shipping and Ordering Information

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Anti-Human Ca_V1.2 (CACNA1C) Antibody