Name: Anti-Human CX3CR1 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ACR-059-F

Overview

Cat #: ACR-059-F

Alternative Name Fractalkine Receptor, CX3C chemokine receptor 1, C-X3-C CKR-1, C-X3-C motif chemokine receptor 1, GPR13, CMKBRL1, GPRV28, V28, G-protein coupled receptor 13, CCRL1, Beta chemokine receptor-like 1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide (C)ESVTENFEYDDLAE, corresponding to amino acid residues 6-19 of human CX3CR1 (Accession P49238). Extracellular, N-terminus.

Accession (Uniprot) Number P49238

Gene ID 1524

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human only

RRID AB_2876847

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN_3.

Isotype Rabbit IgG

Specificity The antibody will not recognize the protein from rat or mouse samples.

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-059), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of CX3CR1 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F), (2.5 µg).
Cell surface detection of CX3CR1 in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F), (2.5 µg).

References

Bronger, H. et al. (2019) Cancer Metastasis Rev. 38, 417.
Hamon, P. et al. (2017) Blood 129, 1296.
Luo, P. et al. (2019) Brain. Res. Bull. 146, 12.
Lee, M. et al. (2018) Immune Netw. 18, e5.
Asai, H. et al. (2015) Nat. Neurosci. 18, 1584.

Scientific background

CX₃C-chemokine receptor 1 (CX₃CR1) is a 368 amino acid (aa) protein that contains seven-transmembrane domains and belongs to the family of G protein coupled receptors¹.

CX₃CR1 has a central role in adhesion and chemotaxis through binding to its ligand, fractalkine CX₃C chemokine ligand 1, (CX₃CL1). The major role of CX₃CR1 in immune cells is to recognize and enter inflamed tissue according to CX₃CL1 gradient and initiate the innate immune system². The receptor-ligand axis also mediates the crosstalk between glial cells and neurons by direct or indirect ways in the central nervous system (CNS)³. Expression and interaction of CX₃CR1 with CX₃CL1 is related to many diseases among which are asthma, cancer and gut infections (reviewed in 4).

Both CX₃CL1 and CX₃CR1 are expressed throughout the body. However, their expression is highly cell type-specific depending on organs and tissues. In the brain, CX₃CL1/CX₃CR1 signaling can modulate the production of cytokines by microglia cells. It has been reported that CX₃CL1/CX₃CR1 signaling is associated with Alzheimer's disease⁵. In the liver, CX₃CR1 is expressed in monocytes, CD8⁺ T cells, and natural killer (NK) cells. Besides immune cells, it is highly expressed at inflammatory sites⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Human CX3CR1 (extracellular) Antibody (#ACR-059) is a highly specific antibody directed against an epitope of human CX3C chemokine receptor 1. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CX3CR1 from human samples only. The antibody will not recognize the protein from rat or mouse samples.

Anti-Human CX3CR1 (extracellular)-FITC Antibody (#ACR-059-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary

Anti-Human CX3CR1 (extracellular)-FITC Antibody