Name: Anti-Human GLUT3 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AGT-043-F

Overview

Cat #: AGT-043-F

Alternative Name Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide (C)KEFINKTLTDKGN, corresponding to amino acid residues 39-51 of human GLUT3 (Accession P11169). 1^st extracellular loop.

Accession (Uniprot) Number P11169

Gene ID 6515

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human only. This antibody will also recognize the human paralog GLUT14 /SLC2A14 (Accession Q8TDB8). Homology – 12/13 amino acid residues identical. The GLUT14 gene is specific to humans and has an overall 94.5% amino acid identity to GLUT3.

RRID AB_2876805

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Specificity The antibody will not recognize rat or mouse GLUT3.

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGT-043), and direct flow cytometry (labeled antibody).

Applications: fc

May also work in: ic*, lci*

Direct flow cytometry

Cell surface detection of GLUT3 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG isotype control-FITC.
^___ Cells + Anti-Human GLUT3 (extracellular)-FITC Antibody (#AGT-043-F), (2.5 µg).

References

Lacombe, V.A. (2014) ISRN Vet. Sci. 2014, 409547.
Simpson, I.A. et al. (2008) Am J. Physiol. 295, E242.
Zheng, C. et al. (2016) Oncol. Lett. 12, 125.

Scientific background

The GLUT protein family is encoded by the SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics^1,2.

GLUT3 is a low-K_m protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called “neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH₂- and COOH- termini. In addition, there are several conserved residues and motifs designated “sugar transporter signatures”^1,2.

GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Human GLUT3 (extracellular) Antibody (#AGT-043) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from human samples only. This antibody will also recognize the human paralog GLUT14 /SLC2A14.

Anti-Human GLUT3 (extracellular)-FITC Antibody (#AGT-043-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-Human GLUT3 (extracellular)-FITC Antibody