Name: Anti-Human GPR132/G2A (extracellular) Antibody
Brand: Alomone Labs
SKU: AGR-046

Overview

Cat #: AGR-046

Alternative Name G protein-coupled receptor 132, G2 accumulation protein, G2A

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide (C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181-195 of human GPR132 (Accession Q9UNW8). 2^ndextracellular loop.

Accession (Uniprot) Number Q9UNW8

Gene ID 29933

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human only.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Specificity The antibody will not recognize rat or mouse samples.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Raji B lymphocyte lymphoma cell line lysate (lanes 2 and 5) and human HL-60 promyelocytic leukemia cell line lysate (lanes 3 and 6):
1-3. Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046), (1:200).
4-6. Anti-Human GPR132/G2A (extracellular) Antibody, preincubated with Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046).

Indirect flow cytometry

Cell surface detection of GPR132 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046), (5 µg) + goat-anti-rabbit-FITC.

References

Weiß, K.T. et al. (2017) Exp. Dermatol. 26, 127.
Justus, C.R. et al. (2013) Front. Physiol. 5, 354.
Okajima, F. (2013) Cell. Signal. 25, 2263.

Scientific background

G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH, and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways¹.

GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis².

G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH-sensing GPCRs are being actively developed and evaluated. Research on pH-sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR132 from human samples only. The antibody will not recognize GPR132 from mouse and rat samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046) is the original antigen used for immunization during Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046) generation. The blocking peptide binds and ‘blocks’ Anti-Human GPR132 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046)

Shipping and Ordering Information

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Anti-Human GPR132/G2A (extracellular) Antibody