Name: Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
Brand: Alomone Labs
SKU: AHR-004

Overview

Cat #: AHR-004

Alternative Name H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4 (Accession Q9H3N8). 1^st extracellular loop.

Accession (Uniprot) Number Q9H3N8

Gene ID 59340

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Won't recognize rat or mouse H4 Histamine Receptor.

RRID AB_2039921.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.7 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates:
1,2. Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).
3,4. Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody, preincubated with Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).

Indirect flow cytometry

Cell surface detection of Histamine H4 receptor by indirect flow cytometry in live intact human HL-60 cell leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (5μg) + goat-anti-rabbit-FITC.
The blocking peptide is not suitable for this application.

References

Hough, L.B. (2001) Mol. Pharmacol. 59, 415.
Thurmund, R.L. et al. (2008) Nat. Rev. Drug. Discov. 7, 41.
Zampeli, E. and Tiligada, E. (2009) Br. J. Pharmacol. 157, 24.

Scientific background

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.¹

The biological effects of histamine are mediated through four receptors (H₁- H₄ receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

Histamine H₄ receptor couples to G_i/G₀ proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.^1,2

Histamine H₄ receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H₄ receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H₄ receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.^2,3

These studies indicate that histamine H₄ receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.^2,3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 1^st extracellular loop of the human histamine H₄ receptor. Anti–Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody (#AHR-004) can be used in western blot and indirect flow cytometry applications, and recognizes H4R from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004) is the original antigen used for immunization during Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody (#AHR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Human Histamine H4 Receptor/HRH4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004)

Shipping and Ordering Information

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Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody