Name: Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody
Brand: Alomone Labs
SKU: AHR-004

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Overview

Cat #: AHR-004

Alternative Name H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4 (Accession Q9H3N8). 1^st extracellular loop.

Accession (Uniprot) Number Q9H3N8

Gene ID 59340

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Won't recognize rat or mouse H4 Histamine Receptor.

RRID AB_2039921.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.7 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Human promyelocytic leukemia (HL-60) and chronic myelogenous leukemia (K562) cell lysates (1:400).
Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates:
1,2. Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).
3,4. Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody, preincubated with Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).

Indirect flow cytometry

Live intact acute T-cell leukemia (Jurkat) cell line (1:20).
The blocking peptide is not suitable for this application.

Scientific background

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.¹

The biological effects of histamine are mediated through four receptors (H₁- H₄ receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

Histamine H₄ receptor couples to G_i/G₀ proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.^1,2

Histamine H₄ receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H₄ receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H₄ receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.^2,3

These studies indicate that histamine H₄ receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.^2,3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004) is the original antigen used for immunization during Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody (#AHR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Human Histamine H4 Receptor/HRH4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human Histamine H4 Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004)

Shipping and Ordering Information

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Certificate of Analysis

Anti-Human Histamine H₄ Receptor (HRH4) (extracellular) Antibody