Specificity Will not recognize rat or mouse LPA5 receptor.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ifc*, ih*, ip*, lci*
Western blot
Human chronic myelogenous leukemia MEG-01 and K562 cell lysates (1:200-1:1000).
Western blot analysis of human chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4):
1,2. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035), (1:200). 3,4. Anti-Human LPAR5 (extracellular) Antibody, preincubated with Human LPAR5 (extracellular) Blocking Peptide (#BLP-LR035).
Scientific background
LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family – LPA1, LPA2, LPA3 and the non-Edg family – LPA4, LPA5, LPA61.
Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.
Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.
LPA5 activation leads to cAMP production; however, it does so in a Gs independent manner4,5. The receptor couples Gq, thus leading to Ca2+ mobilization4.
Activation of LPA5 was also shown to initiate neuropathic pain in mouse models. LPA5 knockout mice do not develop neuropathic pain6.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of human chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4):1,2. Anti-Human LPAR5 (extracellular) Antibody (#ALR-035), (1:200).
