Anti-p75 NGF Receptor/CD271 (ext.)-ATTO Fluor-488 Antibody

Overview

Cat #: ANT-007-AG

Alternative Name p75^NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: H, M, R

Immunogen

Peptide CEEIPGRWITRSTPPE corresponding to amino acid residues 188-203 of human p75^NTR (Accession P08138). Extracellular, stalk domain.

Accession (Uniprot) Number P08138

Gene ID 4804

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; rat - 15/16 amino acid residues identical.

RRID AB_2341009.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-007), and immunocytochemistry (labeled antibody).

Applications: FC, ICC, IF, IHC, LCI

Cited Applications: FC*

Immunohistochemistry

Rat DRG sections.

Direct flow cytometry

Rat pheochromocytoma PC12 cells (1:20).
Cell surface detection of p75^NTR in live intact rat pheochromocytoma (PC12) cells:
^___ Unstained cells.
^___ Cells + Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG), (1:20).

Live cell imaging / Immunocytochemistry

Human A875 melanoma cells (1:50).
Expression of p75^NTR in human A875 melanoma cells
Cell surface detection of p75^NTR in live intact human A875 melanoma cells. A. Cells were stained with Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody (#ANT-007-AG), (1:50), (green). B. Live image of the cells. C. Merge of the two images.

Scientific background

The p75 neurotrophin receptor (p75^NTR) is a member of the tumor necrosis factor (TNF) receptor superfamily. Like all members of this superfamily, p75^NTR is a type I transmembrane protein with tandem cysteine-rich domains in its extracellular portion, as well as an intracellular death domain. p75^NTR binds to its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor, and sortilin. The precise multimeric receptor complex formed between p75^NTR and the other receptors determine the ligand being recognized (see below) and the biological response to its binding^1-3.

As its name implies, p75^NTR binds to all the neurotrophins (NTR) (NGF, BDNF, NT-3, and NT-4) with similar nanomolar affinities. Co-expression of p75^NTR with the Trk receptors enhances its ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75^NTRbinds with high affinity to the unprocessed form of NGF (proNGF), probably as a complex with sortilin, leading to cell death by apoptosis^1-3. Finally, a multimeric complex of p75^NTR and Nogo receptor binds to myelin proteins such as Nogo-66, MAG, and OmgP, resulting in inhibition of axonal growth⁴.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 25/03/2025

Specifications

Citations

Applications

Scientific Background

Specific Control Products

p75 NGF Receptor (extracellular) Blocking Peptide (#BLP-NT007) is the original antigen used for immunization during Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) generation. The blocking peptide binds and ‘blocks’ Anti-p75 NGF Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

p75 NGF Receptor (extracellular) Blocking Peptide (#BLP-NT007)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

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Certificate of Analysis

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody