Overview
- Peptide (C)RPVDGLPPFKMEKTGD, corresponding to amino acid residues 456 - 471 of human TRPV1 (Accession Q8NER1). Extracellular, 1st loop.
Cell surface detection of TRPV1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Human TRPV1 (extracellular)-FITC Antibody (#ACC-334-F), (5µg).
Cell surface detection of TRPV1 by direct flow cytometry in live intact human Jurkat T-Cell leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC.
___ Cells + Anti-Human TRPV1 (extracellular)-FITC Antibody (#ACC-334-F), (5µg) .
TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3
TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids.4 This channel is associated with tissue injury and inflammation.5,6
Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.
There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9
