Overview
- Peptide (C)KQAAPHLKAEVGNS, corresponding to amino acid residues 417-430 of human TRPV2 (Accession Q9Y5S1). 1st extracellular loop.
Western blot analysis of human HMC3 microglial (lanes 1 and 5), Jurkat T-cell leukemia (lanes 2 and 6), HepG2 liver hepatoma (lanes 3 and 7) and chronic myelogenous K562 leukemia (lanes 4 and 8) cell lysates:1-4. Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139), (1:200).
5-8. Anti-Human TRPV2 (VRL1) (extracellular) Antibody, preincubated with Human TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC139).
Cell surface detection of TRPV2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139), (2.5μg) + goat-anti-rabbit-FITC.
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.
According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.
Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.
Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV2 from human samples only. It will not recognize the protein from rat or mouse samples.
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