Name: Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody
Brand: Alomone Labs
SKU: ANT-042

Overview

Cat #: ANT-042

Alternative Name TNFR2, Tumor Necrosis Factor Receptor Superfamily Member 1B, TNFRSF1B, Tumor Necrosis Factor Receptor Type II, TNF-RII, CD120b

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide (C)DQVETQA(S)TREQNR, corresponding to amino acid residues 103 - 116 of human TNFR2 (Accession P20333). Extracellular, N-terminus.

Accession (Uniprot) Number P20333

Gene ID TNFRSF1B

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology The antibody recognizes only human TNFR2.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Specificity Human only

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard quality control of each lot Western blot analysis.

Applications: ifc, lci, wb

May also work in: ih*, ip*

Western blot

Western blot analysis of human HL-60 acute promyelocytic leukemia cell line lysate (lanes 1 and 4), human K562 erythroleukemia cell line lysate (lanes 2 and 5) and human Daudi Burkitt's lymphoma cell line lysate (lanes 3 and 6):
1-3. Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody (#ANT-042), (1:200). 4-6. Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody, preincubated with Human Tumor Necrosis Factor Receptor 2 (extracellular) Blocking Peptide (BLP-NT042).

Indirect flow cytometry

Cell surface detection of TNFR2 by indirect flow cytometry in live intact human
THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody (#ANT-042), (2.5µg) + goat-anti-rabbit-FITC.

References

Medler and Wajant (2019), Expert Opin Ther Targets, 23, 295.
Sheng et al (2018) Front Immunol, 9, 1170.
Yang, S. et al (2018) Front Immunol, 9, 784.

Scientific background

Tumor Necrosis Factor Receptor 2 (TNFR2) is a member of the TNF receptor superfamily (TNFRSF). TNFR2, together with TNF receptor 1 (TNFR1) are the receptors that transduce signals from the pleiotropic cytokine tumor necrosis factor (TNF) ¹.

TNF is a 34 kDa type II transmembrane protein (and soluble variant) important in carcinogenesis and immunity. It was found to either promote or suppress the progression of cancer, depending on the receptor it binds. Signaling through TNFR1 induces strong proinflammatory activities and while TNFR2 has proinflammatory effects too, but it also elicits strong anti-inflammatory activities and has an anti-tumor effect ².

TNFR2 expression is rather restricted to certain cell types, including myeloid cells, regulatory T-cells and glial cells. TNFR2 is also frequently expressed on tumor cells and on suppressive immune cells like myeloid-derived suppressor cells ².

Based on these characteristics, TNFR2 agonists have already shown great potential as drugs for cancer therapy, can potentially exert an effect on heart regeneration, and even neuron regeneration in murine models of Parkinson’s disease ³.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody (#ANT-042) is a highly specific antibody directed against an extracellular epitope of the human TNFRSF1B. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize TNFR2 from human samples only.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Human Tumor Necrosis Factor Receptor 2 (extracellular) Blocking Peptide (#BLP-NT042) is the original antigen used for immunization during Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody (#ANT-042) generation. The blocking peptide binds and ‘blocks’ Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human Tumor Necrosis Factor Receptor 2 (extracellular) Blocking Peptide (#BLP-NT042)

Shipping and Ordering Information

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Anti-Human Tumor Necrosis Factor Receptor 2 (extracellular) Antibody