Overview
- Peptide (C)RQEYRKYGGAKLNR, corresponding to amino acid residues 883-896 of rat IGF1R (Accession P24062). Extracellular, N-terminus (β chain).
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1, 2. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
3, 4. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
4-6. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
Expression of IGF1R in rat cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. IGF1R staining (green) in the parietal cortex is detected in neurons of the pyramidal layer (arrows). B. IGF1R immunoreactivity (green) in the hippocampal CA1 region is observed neurons of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Cell surface detection of IGF1R in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-IGF1R (extracellular) Antibody (#ANT-045), (2.5 µg) + goat-anti-rabbit-FITC.
- Werner, H. et al. (2019) Expert Opin. Investig. Drugs 28, 1101.
- Chughtai, S. (2019) Cell Biochem. Funct. doi. 10.1002/cbf.3479.
- Li, J. et al. (2019) Nat. Commun. 10, 4567.
Insulin-like growth factor receptor (IGF1R) is a cell surface, tyrosine kinase receptor that can be activated by IGF1, IGF2, and insulin1.
IGF receptors are tyrosine kinase-containing heterotetramers that are linked to numerous cytoplasmic signaling cascades and have important roles in various cellular functions. IGF1R displays potent anti-apoptotic, pro-survival capacities and plays a key role in malignant transformation by regulating MAPK and PI3K/AKT signaling pathways. IGF1R regulators are identified as a candidate therapeutic targets in cancer and growth-related diseases1,2.
IGF1R and insulin receptor (IR) are closely related, and share 57% sequence identity and high structural similarity. Each IGF1R and IR receptor consists of the L1, CR, L2, FnIII-1,-2,-3, transmembrane (TM), and kinase domain. Two such protomers are linked by multiple disulfide bonds, forming a stable, covalent dimer. Upon ligand binding, the dimer undergoes significant conformational changes which facilitate its further signal transduction cascade3.
Anti-IGF1R (extracellular) Antibody (#ANT-045) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize IGF1R from mouse, rat, and human samples.
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