Name: Anti-IQGAP1 Antibody
Brand: Alomone Labs
SKU: APZ-055

Overview

Cat #: APZ-055

Alternative Name IQ Motif Containing GTPase Activating Protein 1, Ras GTPase-Activating-Like Protein IQGAP1, p195

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KAYQDRLAYLRSHKDE, corresponding to amino acid residues 793 - 808 of human IQGAP1 (Accession P46940). Intracellular.

Accession (Uniprot) Number P46940

Gene ID 8826

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 15/16 amino acid residues identical.

RRID AB_2876830

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
3, 4. Anti-IQGAP1 Antibody, preincubated with IQGAP1 Blocking Peptide (#BLP-PZ055) (#BLP-PZ055).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 2 and 4):
1, 2. Anti-IQGAP1 Antibody (#APZ-055), (1:200).
3, 4. Anti-IQGAP1 Antibody, preincubated with IQGAP1 Blocking Peptide (#BLP-PZ055) (#BLP-PZ055).

Immunohistochemistry

Expression of IQGAP1 in mouse cerebellum
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-IQGAP1 Antibody (#APZ-055), (1:200), followed by goat anti-rabbit-AlexaFluor-488. IQGAP1 immunoreactivity appears in Purkinje neurons (horizontal arrows) and their dendrites (vertical arrows). Cell nuclei are stained with DAPI (blue).

References

Abel, A.M. et al. (2015) Mol. Immunol. 65, 336.
Li, Z. et al. (2005) J. Biol. Chem. 280, 13871.
Hedman, A.C. et al. (2015) EMBO Rep. 16, 427.
Gao, C. et al. (2011) J. Neurosci. 31, 8533.
Johnson, M. et al. (2011) Int. J. Biochem. Cell Biol. 43, 65.
Johnson, M. et al. (2009) Cell. Signal. 21, 1471.

Scientific background

IQGAP1 is a 190 kDa (1657 amino acid) protein that is ubiquitously expressed and contains six distinct protein-interacting domains with six coiled coil regions¹.

The Ras GTPase-activating-like protein is a scaffold protein involved in regulating various cellular processes; from organizing the actin cytoskeleton, transcription, cell adhesion, and regulating the cell cycle^2,3.

IQGAP1 binds to activated CDC42 but does not stimulate its GTPase activity and it may have a role in cell cycle progression following DNA damage^1,2. The protein also regulates NR2A NMDA receptor signaling where it influences cognitive processes⁴. It also regulates MAPK and Wnt/β-catenin signaling pathways⁵. It is thought to contribute to tumorigenesis by stimulating cell motility and invasion⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-IQGAP1 Antibody (#APZ-055) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize IQGAP1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

IQGAP1 Blocking Peptide (#BLP-PZ055) is the original antigen used for immunization during Anti-IQGAP1 Antibody (#APZ-055) generation. The blocking peptide binds and ‘blocks’ Anti-IQGAP1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

IQGAP1 Blocking Peptide (#BLP-PZ055)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-IQGAP1 Antibody