Overview
- Peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1 (Accession O00180). Extracellular, near the P1 loop.
Western blot analysis of rat brain lysate:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:200).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110).
Western blot analysis of HEK-293-KCNK1 transfected cells:1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:300).
2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110).
Expression of KCNK1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110). A. KCNK1 channel appears in glial processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK1 channel and calbindin D28K demonstrates the separate localization of these proteins.
- Lesage, F. et al. (1996) EMBO J. 15, 1004.
- Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.
- Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175.
KCNK1 (also named TWIK-1 or K2P1.1) is a member of the 2-pore (2P) domain K+ channels family that, currently, includes 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini.
K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical K+ channel blockers.
KCNK1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human K2P1.1 channel. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting KCNK1 in living cells. It has been designed to recognize KCNK1 from mouse, rat and human samples.
Applications
Citations
Powered by Bioz- Mouse primary cortical astrocyte lysate (1:500).
Hwang, E.M. et al. (2014) Nat. Commun. 5, 3227.
- Pivonkova, H. et al. (2010) Neurochem. Int. 57, 783.
- Zhou, M. et al. (2009) J. Neurosci. 29, 8551.
