Name: Anti-KCNK15 (TASK-5) (extracellular) Antibody
Brand: Alomone Labs
SKU: APC-158

Overview

Cat #: APC-158

Alternative Name Potassium channel subfamily K member 15, Acid-sensitive K⁺ channel protein TASK-5, TWIK-related acid-sensitive K⁺ channel 5, K_2P15.1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

Immunogen

Peptide (C)RKRGEFRRKYRFSAD, corresponding to amino acid residues 43-57 of rat KCNK15 (Accession Q8R5I0). 1^st extracellular loop.

Accession (Uniprot) Number Q8R5I0

Gene ID 156873

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse – identical.

RRID AB_2756747.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity Not recommended for human samples

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*, lci*

Western blot

Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):
1,2. Anti-KCNK15 (TASK-5) (extracellular) Antibody (#APC-158), (1:200).
3,4. Anti-KCNK15 (TASK-5) (extracellular) Antibody, preincubated with KCNK15/TASK-5 (extracellular) Blocking Peptide (#BLP-PC158).

References

Kim, D. and Gnatenco, C. (2001) Biochem. Biophys. Res. Commun. 284, 923.
Goldstein, S.A. et al. (2005) Pharmacol. Rev. 57, 527.

Scientific background

TASK1-5 are members of the two-pore domain K⁺ channels. They have four transmembrane domains and assist in setting the resting membrane potential.

TASK5 (K_2P15.1, KCNK15) was identified using the TASK-3 sequence in a GenBank search. However, when expressed in COS-7 cells, it does not form a functional K⁺ current¹.

The channel is distributed in the brain, heart, lung, kidney, liver, pancreas, adrenal gland and placenta².

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat KCNK15 channel. Anti-KCNK15 (TASK-5) (extracellular) Antibody (#APC-158) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KCNK15 from rat and mouse samples. The antibody will unlikely recognize the channel from human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

KCNK15/TASK-5 (extracellular) Blocking Peptide (#BLP-PC158) is the original antigen used for immunization during Anti-KCNK15 (TASK-5) (extracellular) Antibody (#APC-158) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK15/TASK-5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNK15/TASK-5 (extracellular) Blocking Peptide (#BLP-PC158)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-KCNK15 (TASK-5) (extracellular) Antibody