Overview
Alternative Name Potassium channel subfamily K member 15, Acid-sensitive K+ channel protein TASK-5, TWIK-related acid-sensitive K+ channel 5, K2P15.1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
- Peptide (C)RKRGEFRRKYRFSAD, corresponding to amino acid residues 43-57 of rat KCNK15 (Accession Q8R5I0). 1st extracellular loop.
Accession (Uniprot) Number Q8R5I0
Gene ID 156873
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical.
RRID AB_2756747.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Specificity Not recommended for human samples
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
- Rat and mouse brain lysates (1:200-1:1000).
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain lysates (lanes 2 and 4):1,2. Anti-KCNK15 (TASK-5) (extracellular) Antibody (#APC-158), (1:200).
3,4. Anti-KCNK15 (TASK-5) (extracellular) Antibody, preincubated with KCNK15/TASK-5 (extracellular) Blocking Peptide (#BLP-PC158).
Scientific background
TASK1-5 are members of the two-pore domain K+ channels. They have four transmembrane domains and assist in setting the resting membrane potential.
TASK5 (K2P15.1, KCNK15) was identified using the TASK-3 sequence in a GenBank search. However, when expressed in COS-7 cells, it does not form a functional K+ current1.
The channel is distributed in the brain, heart, lung, kidney, liver, pancreas, adrenal gland and placenta2.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
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