Name: Anti-KCNK6 (TWIK-2) Antibody
Brand: Alomone Labs
SKU: APC-040

Overview

Cat #: APC-040

Alternative Name Potassium channel subfamily K member 6, TOSS, K_2P6.1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, r

Immunogen

Peptide (C)ESHQQLSASSHTDYASIPR, corresponding to residues 295-313 of human KCNK6 (Accession Q9Y257). Intracellular, C-terminus.

Accession (Uniprot) Number Q9Y257

Gene ID 9424

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat - 13/17 amino acid residues identical.

RRID AB_2040142.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.6 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-KCNK6 (TWIK-2) Antibody (#APC-040), (1:200).
2. Anti-KCNK6 (TWIK-2) Antibody, preincubated with KCNK6/TWIK-2 Blocking Peptide (#BLP-PC040).

References

Chavez, R.A. et al. (1999) J. Biol. Chem. 274, 7887.
Patel, A.J. et al. (2000) J. Biol. Chem. 275, 28722.
Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793.

Scientific background

KCNK6 (also named Tandem of P domain of Weak Inward rectifier K⁺ channel 2, TWIK-2 or K_2P6.1) is a member of the 2-pore (2P) domain K⁺ channel family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K⁺ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.

The K_2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.

K_2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.

KCNK6 displays low single channel conductance of about 5pS and is partially activated by arachidonic acid and blocked by volatile anesthetics.

KCNK6 expression is widespread, especially in the rat were it is expressed in brain, heart, kidney and liver. In humans KCNK6 expression is more restricted with expression in aorta, stomach, placenta and spleen while in mouse, KCNK6 expression has been detected only in liver.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-KCNK6 (TWIK-2) Antibody (#APC-040) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize KCNK6 from rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

More product citations

Lloyd, E.E. et al. (2009) Exp. Biol. Med. 234, 1493.
Gardener, M.J. et al. (2004) Br. J. Pharmacol. 142, 192.

Specifications

Scientific Background

Specific Control Product

KCNK6/TWIK-2 Blocking Peptide (#BLP-PC040) is the original antigen used for immunization during Anti-KCNK6 (TWIK-2) Antibody (#APC-040) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK6/TWIK-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNK6/TWIK-2 Blocking Peptide (#BLP-PC040)

Shipping and Ordering Information

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Anti-KCNK6 (TWIK-2) Antibody