Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
Rat cerebellum lysate (1:200).
Western blot analysis of rat cerebellum lysate:
1. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044), (1:200). 2. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with the control peptide antigen.
Immunohistochemistry
Rat brain sections (Okamoto, K.et al. (2016) eNeuro3, e0138-16.2016.).
Scientific background
KCNK9 (also named TASK3 or K2P9.1) is a member of the 2-pore (2P) domain K+ channels family that probably assemble as dimers to constitute a functional channel.1 These channels show little time- or voltage-dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.
The channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc.2 The physiological role of the K2P channels is not yet clear, though they were proposed to participate in breathing, aldosterone secretion and anesthetic-mediated neuronal activity.
In human, expression of KCNK9 is restricted in brain and its expression overlaps with the structurally related KCNK3 (TASK1, K2P3.1) channel. In rat, it is more widely expressed in other tissues as well as in brain.
Unexpectedly, KCNK9 was found to be over-expressed between 5 to 100-fold in 44% of breast cancer tumors tested. Transfection and over-expression of KCNK9 in cell lines promotes tumor growth and confers resistance to hypoxia and serum deprivation.3 Recently, KCNK9 was found to play an important role in K+-dependent apoptosis of cerebellar granules.4
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot