Overview
- Peptide (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606). Intracellular, N-terminal part.
Western blot analysis of rat brain cortex:1. Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200).
2. Anti-KCNN1 (KCa2.1, SK1) Antibody, preincubated with KCNN1/KCa2.1 Blocking Peptide (#BLP-PC039).- Mouse isolated mouse atria and HL-1 cell lysate (1:200) (Yi, F. et al. (2015) J. Biol. Chem. 290, 7016.).
- Mouse kidney sections (1:200) (Li, Y. et al. (2016) PLoS ONE 11, e0155006.).
Expression of KCa2.1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. KCa2.1 immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with KCNN1 (KCa2.1, SK1) Blocking Peptide (BLP-PC039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of KCa2.1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region shows KCa2.1 immunoreactivity (green) in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with KCNN1 (KCa2.1, SK1) Blocking Peptide (BLP-PC039), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Mouse CCDcl1 cells (1:100) (Li, Y. et al. (2016) PLoS ONE 11, e0155006.).
- Sailer, C.A. et al. (2002) J. Neurosci. 22, 9698.
- Monaghan, A.S. et al. (2004) J. Biol. Chem. 279, 1003.
KCa2.1 is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is, six transmembrane domains and intracellular N- and C-termini.
KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well.
The sensitivity of KCa2.1 to the bee venom toxin Apamin (#STA-200) is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to Apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.
Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN1 from human, rat, and mouse samples.
Applications
Citations
Powered by Bioz- Mouse brain lysate.
Deng, P.Y. et al. (2019) J. Neurosci. 39, 28. - Rat brain lysate (1:500).
Larson, R.A. et al. (2015) Am. J. Physiol. 308, H1547. - Mouse isolated mouse atria and HL-1 cell lysate (1:200).
Yi, F. et al. (2015) J. Biol. Chem. 290, 7016. - Canine LA and PV cardiomyocytes (1:200).
Qi, X.Y. et al. (2014) Circulation 129, 430.
- Mouse kidney sections (1:200).
Li, Y. et al. (2016) PLoS ONE 11, e0155006.
- Mouse CCDcl1 cells (1:100).
Li, Y. et al. (2016) PLoS ONE 11, e0155006.
- Gui, L. et al. (2013) Am. J. Physiol. 304, H118.
- Wang, W. et al. (2011) Neuropharmacology 60, 901.
- Andres, M.A. et al. (2009) FEBS Lett. 583, 369.
- N'Gouemo, P. et al. (2009) Brain Res. 1270, 107.
