Overview
- Peptide (C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2 (Accession P63161). Intracellular, C-terminal domain.
Western blot analysis of rat heart membranes:1. Anti-KCNE2 (MiRP1) Antibody (#APC-054), (1:200).
2. Anti-KCNE2 (MiRP1) Antibody, preincubated with KCNE2/MiRP1 Blocking Peptide (#BLP-PC054).- Human ventricle lysate (Zhang, M. et al. (2012) Am. J. Physiol. 302, H910.).
- Rat sinoatrial cells (SANs) (Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.).
Expression of KCNE2 in mouse heartImmunohistochemical staining of mouse heart frozen sections using Anti-KCNE2 (MiRP1) Antibody (#APC-054), (red). Immunoreactivity appears as a striated pattern in heart muscle.
- Rabbit carotid body chemoreceptor cells (Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.).
- Abbott, G.W. et al. (1999) Cell 97, 175.
- Abbott, G.W. and Goldstein S.A. (2001) Mol. Interv. 1, 95.
- Roepke, T.K. et al. (2006) J. Biol. Chem. 281, 23740.
KCNE2 (or MiRP1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE5 (MiRP4).
KCNE1 is the founding member of the family and KCNE2 was discovered based on its homology with KCNE1.1,2
The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.1,2
KCNE2 is broadly distributed with prominent expression in the brain and heart, but also in other tissues such as skeletal muscle, pancreas and kidney.
The best described functional association of KCNE2 is with the voltage-gated K+ channel KV11.1 (HERG). In the heart, complexes of the two proteins constitute the molecular underlay of the cardiac IKr current and mutations in the KCNE2 subunit are associated with cardiac arrhythmias.1 KCNE2 can form complexes with other channels including the K+ channels KV7.1 (KCNQ1) and KV4.3 and the hyperpolarization-activated cation channels HCN1 and HCN2. In the stomach KV7.1/KCNE2 complexes were found to be crucial for acid secretion as well as for the maintenance of structural integrity, and for parietal cell survival.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNE1. Anti-KCNE2 (MiRP1) Antibody (#APC-054) can be used in western blot, immunohistochemistry, immunocytochemistry and immunoprecipitation applications. It has been designed to recognize KCNE2 from rat, mouse and human samples.
Applications
Citations
Powered by Bioz- Rat ventricular myocytes (1:200).
Liu, W.J. et al. (2015) Am. J. Physiol. 309, H1288. - Cultured mouse primary cortical/hippocampal neurons (1:250).
Sachse, C.C. et al. (2013) FASEB J. 27, 2458. - Human ventricle lysate.
Zhang, M. et al. (2012) Am. J. Physiol. 302, H910.
- Rat sinoatrial cells (SANs).
Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.
- Rabbit carotid body chemoreceptor cells.
Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.
