Name: Anti-KCNQ1 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: APC-168-F

Overview

Cat #: APC-168-F

Alternative Name K_V7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1 (Accession Q9Z0N7). 3^rd extracellular loop.

Accession (Uniprot) Number Q9Z0N7

Gene ID 84020

Homology Mouse – identical; human – 13/14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-168), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of KCNQ1 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F), (2.5µg).
Cell surface detection of KCNQ1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F), (5µg).

References

Wang, Q. et al. (1996) Nat. Genet. 12, 17.
Grahammer, F. et al. (2001) Gastroenterology 120, 1363.
Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.

Scientific background

KCNQ1 (K_V7.1) is part of a voltage-gated K⁺ family that includes 5 other members (KCNQ1-5).

The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac I_ks current that controls the duration of the action potential of the human heart. Indeed mutations on either K_V7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death.

Although K_V7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl^- secretion into the colon.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-KCNQ1 (extracellular) Antibody (#APC-168) is a highly specific antibody directed against an extracellular epitope of the rat KCNQ1 (K_V7.1). The antibody can be used in western blot, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize KCNQ1 from mouse, rat and human samples.

Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize KCNQ1 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

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Anti-KCNQ1 (extracellular)-FITC Antibody