Overview
Alternative Name KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1 (Accession Q9Z0N7). 3rd extracellular loop.
Accession (Uniprot) Number Q9Z0N7
Gene ID 84020
Homology Mouse – identical; human – 13/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APC-168), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
Cell surface detection of KCNQ1 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F), (2.5µg).
Cell surface detection of KCNQ1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F), (5µg).
Scientific background
KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).
The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death.
Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl- secretion into the colon.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 07/05/2024
Specifications
Citations
Citations
