Name: Anti-KCNS2 (KV9.2) Antibody
Brand: Alomone Labs
SKU: APC-132

Overview

Cat #: APC-132

Alternative Name Voltage-gated K⁺ channel subunit K_V9.2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CDFGDGMKEVPSVNLR, corresponding to amino acid residues 430-445 of rat K_V9.2 (Accession Q9ER26). Intracellular, C-terminus.

Accession (Uniprot) Number Q9ER26

Gene ID 66022

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ifc*, ih*, ip*

Western blot

Western blot analysis of mouse brain lysate:
1. Anti-KCNS2 (K_V9.2) Antibody (#APC-132), (1:200).
2. Anti-KCNS2 (K_V9.2) Antibody, preincubated with KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132).
Western blot analysis of rat brain lysate:
1. Anti-KCNS2 (K_V9.2) Antibody (#APC-132), (1:200).
2. Anti-KCNS2 (K_V9.2) Antibody, preincubated with KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132).

References

Salina, M. et al. (1997) J. Biol. Chem. 272, 24371.
Bocksteins, E. et al. (2016) J. Gen. Physiol. 147, 105.

Scientific background

K_V9.2 is a member of the electrically silent voltage-gated K⁺ (K_V). Ten K_V silent subunits have been identified: K_V5.1, K_V6.1–K_V6.4, K_V8.1, K_V8.2, and K_V9.1–K_V9.3. K_V9.2 modulates the activity of K_V2.1 and K_V2.2 channels^1,2. K_V9.2 structure includes six transmembrane segments: S1–S6, positive residues on S4, the K⁺ signature sequence GYG on the pore loop between S5 and S6, and cytoplasmic N and C termini².

High K_V9.2 expression level can be found in the olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei, and cerebellum¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-KCNS2 (K_V9.2) Antibody (#APC-132) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize K_V9.2 channel from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132) is the original antigen used for immunization during Anti-KCNS2 (K_V9.2) Antibody (#APC-132) generation. The blocking peptide binds and ‘blocks’ Anti-KCNS2/Kv9.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

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Anti-KCNS2 (K_V9.2) Antibody