Overview
Alternative Name Voltage-gated K+ channel subunit KV9.2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide CDFGDGMKEVPSVNLR, corresponding to amino acid residues 430-445 of rat KV9.2 (Accession Q9ER26). Intracellular, C-terminus.
Accession (Uniprot) Number Q9ER26
Gene ID 66022
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
- Mouse and rat brain lysates (1:200-1:1000).
Western blot analysis of mouse brain lysate:1. Anti-KCNS2 (KV9.2) Antibody (#APC-132), (1:200).
2. Anti-KCNS2 (KV9.2) Antibody, preincubated with KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132).
Western blot analysis of rat brain lysate:1. Anti-KCNS2 (KV9.2) Antibody (#APC-132), (1:200).
2. Anti-KCNS2 (KV9.2) Antibody, preincubated with KCNS2/Kv9.2 Blocking Peptide (#BLP-PC132).
Scientific background
KV9.2 is a member of the electrically silent voltage-gated K+ (KV). Ten KV silent subunits have been identified: KV5.1, KV6.1–KV6.4, KV8.1, KV8.2, and KV9.1–KV9.3. KV9.2 modulates the activity of KV2.1 and KV2.2 channels1,2. KV9.2 structure includes six transmembrane segments: S1–S6, positive residues on S4, the K+ signature sequence GYG on the pore loop between S5 and S6, and cytoplasmic N and C termini2.
High KV9.2 expression level can be found in the olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei, and cerebellum1.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 29/10/2024
Specifications
Citations
Citations
