Name: Anti-Kir2.3 (KCNJ4) Antibody
Brand: Alomone Labs
SKU: APC-032

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Overview

Cat #: APC-032

Alternative Name Inward rectifier potassium channel 4, BIR11, IRK3, HIRK2, HRK1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EFGS HLDLE RMQAA TLPLD N, corresponding to amino acid residues 418-437 of rat K_ir2.3 (Accession P52190). Intracellular, C-terminal part.

Accession (Uniprot) Number P52190

Gene ID 116649

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; guinea pig - 19/20 amino acid residues identical; hamster, human, Xenopus - 18/20 amino acid residues identical.

RRID AB_2040111.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Rat brain membranes (1:200).

Human heart lysate (1:200) (Voigt, N. et al. (2010) Circ. Arrhyth. Electrophysiol. 3, 472.).
Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes:
1,2. Anti-K_ir2.3 (KCNJ4) Antibody (#APC-032), (1:200)
3,4. Anti-K_ir2.3 (KCNJ4) Antibody, preincubated with Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032).

Immunohistochemistry

Rat brain sections.

Immunocytochemistry

Canine ventricle and atrium myocytes (1:300) (Melnyk, P. et al. (2002) Am. J. Physiol. 283, H1123.).

Scientific background

K_ir2.3 is a member of the family of inward rectifying K⁺ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K⁺ channels.

The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the K_ir channels is composed of four subunit that can assembly as either homo or heterotetramers.

K_ir channels are characterized by a K⁺ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K⁺ homeostasis.

K_ir2.3 is a member of the K_ir2.x subfamily that includes four members (K_ir2.1- K_ir2.4) that are characterized by strong inward rectification and high constitutive activity.

K_ir2.3 is expressed in a variety of tissues including heart and brain. ²

In the heart, K_ir2.3 forms heteromers with K_ir2.1 and underlay the I_K1 current (at least in some species) that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na⁺ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.^1,2

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title: Anti-Kir2.3 (KCNJ4) Antibody

Expression of K_ir2.1 and K_ir2.3 channels in Lamina I pacemaker cells.Immunohistochemical staining of rat spinal cord sections using Lamina I pacemaker using Anti-K_ir2.1 (KCNJ2) Antibody (#APC-026) and Anti-K_ir2.3 (KCNJ4) Antibody (#APC-032). Confocal images of representative biocytin-filled pacemaker neurons (red) processed for K_ir (green). Merged images demonstrate that immunoreactive puncta for K_ir2.1 and K_ir2.3 (yellow; inset) are localized to identified pacemaker neurons within lamina I of the neonatal spinal cord (right). Scale bars: 10 μm; inset, 2 μm.Adapted from Li, J. et al. (2013) with permission of The Society for Neuroscience.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032) is the original antigen used for immunization during Anti-K_ir2.3 (KCNJ4) Antibody (#APC-032) generation. The blocking peptide binds and ‘blocks’ Anti-Kir2.3/KCNJ4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032)

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Anti-K_ir2.3 (KCNJ4) Antibody