Overview
- Peptide (C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat KCNJ9 (Accession Q63511). Intracellular, C-terminus.
Western blot analysis of rat brain membranes:1. Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:200).
2. Anti-GIRK3 (Kir3.3) Antibody, preincubated with GIRK3/Kir3.3 Blocking Peptide (#BLP-PC038).
- Rat brain sections.
Expression of GIRK3 in mouse deep cerebellar nuclei (DCN).Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:400), followed by goat anti-rabbit-Alexa-488. A. GIRK3 immunoreactivity (green) appears in DCN cells (arrows). B. Pre-incubation of the antibody with GIRK3 (Kir3.3) Blocking Peptide (#BLP-PC038), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Mouse INS-IE cells (Iwanir, S. and Reuveny, E. (2008) Pflugers Arch. 456, 1097.).
- Dascal, N. (1997) Cell Signal 9, 551.
- Mark, M.D. et al. (2000) Eur. J. Biochem. 267, 5830.
- Torrecilla, M. et al. (2002) J. Neurosci. 22, 4328.
Kir3.3 (or G-protein regulated Inward-Rectifier K+ channel, GIRK3) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.
The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers.
Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.
Kir3.3 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Ga-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.1,2
Kir3.3 is mainly expressed in the brain, were it co-assembles with Kir3.1 or Kir3.2. The functional impact of Kir3.3 is less well understood than the other Kir3 channels. However, heteromers composed of Kir3.2 and Kir3.3 were found to be primarily responsible for the opioid-induced current and hyperpolarization observed in mouse locus ceruleus (LC) neurons.3
Anti-GIRK3 (Kir3.3) Antibody (#APC-038) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir3.3 from human, rat, and mouse samples.
Applications
Citations
Powered by Bioz- Western blot analysis of mouse brain lysate. Tested in GIRK3-/- mice.
Koyrakh, L. et al. (2005) J. Neurosci. 25, 11468.
- Mouse brain lysate. Also tested in GIRK3-/- mice.
Koyrakh, L. et al. (2005) J. Neurosci. 25, 11468.
- Mouse pancreas sections.
Iwanir, S. and Reuveny, E. (2008) Pflugers Arch. 456, 1097.
- Mouse INS-IE cells.
Iwanir, S. and Reuveny, E. (2008) Pflugers Arch. 456, 1097.
- Booker, S.A. et al. (2016) Cereb. Cortex pii: bhw090.
- Del Burgo, L.S. et al. (2008) J. Comp. Neurol. 510, 581.
