Name: Anti-KCNJ15 (Kir4.2) Antibody
Brand: Alomone Labs
SKU: APC-058

Overview

Cat #: APC-058

Alternative Name ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, K_ir1.3, IRKK

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15 (Accession O88932). Intracellular, C-terminal part.

Accession (Uniprot) Number O88932

Gene ID 16516

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Guinea pig - 19/20 amino acid residues identical; human - 18/20 amino acid residues identical.

RRID AB_2040122.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.6 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of rat kidney membranes:
1. Anti-KCNJ15 (K_ir4.2) Antibody (#APC-058), (1:200).
2. Anti-KCNJ15 (K_ir4.2) Antibody, preincubated with KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058).
Human retina lysate (Yang, D. et al. (2008) Exp. Eye Res. 87, 176.).

Immunohistochemistry

Rat retina sections (1:100) (Tian, M. et al. (2003) Neurosci. Lett. 345, 9.).

References

Hill, C.E. et al. (2002) Am. J. Physiol. 282, G233.
Pessia, M. et al. (2001) J. Physiol. 532, 359.

Scientific background

K_ir4.2 is a member of the family of inward rectifying K⁺ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K⁺ channels.

The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K⁺ channels the functional unit for the K_ir channels is composed of four subunit that can assembly as either homo or heteromers.

K_ir channels are characterized by a K⁺ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K⁺ homeostasis.

K_ir4.2 is a member of the K_ir4 subfamily that includes one other member: K_ir4.1. K_ir4.2 can co-assemble with K_ir4.1 but also with other K_ir channels such as K_ir1.1 and K_ir5.1.²

The K_ir4 subfamily has been classified as weak rectifiers with intermediate conductance.

K_ir4.2 is expressed in liver, kidney, pancreas lung and testis. Its physiological function is not well understood but it has been suggested that it could be involved in the regulation of K⁺ efflux in epithelial cells such as hepatocytes or lung cells.¹

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse K_ir4.2 channel. Anti-KCNJ15 (K_ir4.2) Antibody (#APC-058) can be used in western blot and immunohistochemistry applications. It has been designed to recognize K_ir4.2 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Human retina lysate.
Yang, D. et al. (2008) Exp. Eye Res. 87, 176.

Immunohistochemistry citations

Rat retina sections (1:100).
Tian, M. et al. (2003) Neurosci. Lett. 345, 9.

More product citations

Prüss, H. et al. (2011) Neuroscience 180, 19.

Specifications

Scientific Background

Specific Control Product

KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058) is the original antigen used for immunization during Anti-KCNJ15 (K_ir4.2) Antibody (#APC-058) generation. The blocking peptide binds and ‘blocks’ Anti-KCNJ15/Kir4.2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058)

Shipping and Ordering Information

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Anti-KCNJ15 (K_ir4.2) Antibody