Name: Anti-Kir6.1 (KCNJ8) Antibody
Brand: Alomone Labs
SKU: APC-105

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Overview

Cat #: APC-105

Alternative Name ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat K_ir6.1 (Accession Q63664). Intracellular, C-terminal part.

Accession (Uniprot) Number Q63664

Gene ID 25472

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human, pig, rabbit - 15/16 amino acid residues identical.

RRID AB_2039945.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Specificity The antibody is specific for K_ir6.1 and does not cross react with K_ir6.2.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, ip, wb

May also work in: ic*, ifc*

Western blot

Rat heart membranes and rat cortex lysate (1:200).

Human umbilical vein segment lysate (Protić, D. et al. (2014) Phytother. Res. 28, 1412.).
Western blot analysis of rat heart membranes:
1. Anti-K_ir6.1 (KCNJ8) Antibody (#APC-105), (1:200).
2. Anti-K_ir6.1 (KCNJ8) Antibody, preincubated with Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105).
Western blot analysis of rat cortex lysate:
1. Anti-K_ir6.1 (KCNJ8) Antibody (#APC-105), (1:200).
2. Anti-K_ir6.1 (KCNJ8) Antibody, preincubated with Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105).

Immunoprecipitation

Mouse heart lysate (Li, J. et al. (2010) J. Biol. Chem. 285, 28723.).

Immunohistochemistry

Rat uterus sections (1:50).

Mouse brain sections (1:100) (Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061.).

Scientific background

K_ir6.1 is a member of the inward rectifier K⁺ channels (K_ir channels), a large family of voltage-independent K⁺ channels largely involved in stabilization of the membrane resting potential and in K⁺ transport across membranes.

K_ir6.1, like its close relative K_ir6.2, is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization thereby coupling intracellular metabolism to cellular excitability.

K_ir6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N- and C-termini located intracellularly.

The functional ATP sensitive channel (K_ATP) is composed of octamers of four K_ir6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B.

The channel's tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Anti-Kir6.1 (KCNJ8) Antibody Expression of K_ir6.1 in mouse brain sections.Immunohistochemical staining of mouse brain sections using Anti-K_ir6.1 (KCNJ8) Antibody (#APC-105). K_ir6.1 staining (green) is detected and coincides with orexin neurons.Adapted from Parsons, M.P. and Hirasawa, M. (2010) J. Neurosci. 30, 8061. with permission of the Society for Neuroscience.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105) is the original antigen used for immunization during Anti-K_ir6.1 (KCNJ8) Antibody (#APC-105) generation. The blocking peptide binds and ‘blocks’ Anti-Kir6.1/KCNJ8 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105)

Shipping and Ordering Information

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Certificate of Analysis

Anti-K_ir6.1 (KCNJ8) Antibody